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(A) Activated intracellular kinases were analyzed by using the Human Phospho-Kinase Array Kit. Whole-cell extracts from H23 cells, treated or not treated (Mock) with an <t>IL6</t> family cytokine (rhIL6, rhOSM, or rhLIF) for 48 hours were incubated with the arrays, and phosphorylation status of each kinase was determined.
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(A) Activated intracellular kinases were analyzed by using the Human Phospho-Kinase Array Kit. Whole-cell extracts from H23 cells, treated or not treated (Mock) with an IL6 family cytokine (rhIL6, rhOSM, or rhLIF) for 48 hours were incubated with the arrays, and phosphorylation status of each kinase was determined.

Journal: Molecular cancer therapeutics

Article Title: JAK1/STAT3 activation through a proinflammatory cytokine pathway leads to resistance to molecularly targeted therapy in non-small cell lung cancer

doi: 10.1158/1535-7163.MCT-17-0148

Figure Lengend Snippet: (A) Activated intracellular kinases were analyzed by using the Human Phospho-Kinase Array Kit. Whole-cell extracts from H23 cells, treated or not treated (Mock) with an IL6 family cytokine (rhIL6, rhOSM, or rhLIF) for 48 hours were incubated with the arrays, and phosphorylation status of each kinase was determined.

Article Snippet: Recombinant human (rh) IL6, OSM, and LIF proteins were purchased from EMD Millipore.

Techniques: Incubation

(A) (Upper) Relative expression levels of IL6 and OSM mRNA in normal lung fibroblasts (NLFs) and cancer-associated fibroblasts (CAFs) compared to HBEC3KT cells were analyzed by qRT-PCR. (Lower) Levels of IL6 protein in cell culture supernatants and OSM protein in whole-cell lysates from HBEC3KT, NLFs, and CAFs were analyzed by ELISA.

Journal: Molecular cancer therapeutics

Article Title: JAK1/STAT3 activation through a proinflammatory cytokine pathway leads to resistance to molecularly targeted therapy in non-small cell lung cancer

doi: 10.1158/1535-7163.MCT-17-0148

Figure Lengend Snippet: (A) (Upper) Relative expression levels of IL6 and OSM mRNA in normal lung fibroblasts (NLFs) and cancer-associated fibroblasts (CAFs) compared to HBEC3KT cells were analyzed by qRT-PCR. (Lower) Levels of IL6 protein in cell culture supernatants and OSM protein in whole-cell lysates from HBEC3KT, NLFs, and CAFs were analyzed by ELISA.

Article Snippet: Recombinant human (rh) IL6, OSM, and LIF proteins were purchased from EMD Millipore.

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Enzyme-linked Immunosorbent Assay

(A) The correlation between expression of each IL6 family cytokine and receptor in TCGA lung adenocarcinoma samples. Spearman ρ value is plotted in each box.

Journal: Molecular cancer therapeutics

Article Title: JAK1/STAT3 activation through a proinflammatory cytokine pathway leads to resistance to molecularly targeted therapy in non-small cell lung cancer

doi: 10.1158/1535-7163.MCT-17-0148

Figure Lengend Snippet: (A) The correlation between expression of each IL6 family cytokine and receptor in TCGA lung adenocarcinoma samples. Spearman ρ value is plotted in each box.

Article Snippet: Recombinant human (rh) IL6, OSM, and LIF proteins were purchased from EMD Millipore.

Techniques: Expressing